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. 2017 Aug 14;11:239. doi: 10.3389/fncel.2017.00239

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Copyright © 2017 Bitzenhofer, Ahlbeck and Hanganu-Opatz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Optogenetic activation of pyramidal neurons in the neonatal PFC by different patterns of light stimulation in vivo. (Ai) Representative raster plot and spike probability histogram displaying the firing of ChR2(ET/TC) transfected layer II/III pyramidal neurons in response to 30 sweeps of repetitive light pulses (473 nm, 3 ms, 14 mW/mm²) at 8 Hz recorded from a neonatal mouse after IUE at E15.5. (ii) Corresponding inter-spike interval (ISI) histogram averaged for 3 s before light stimulation (pre, black), 3 s during ramp stimulation (stimulation, blue), and 3 s after light stimulation (post, gray). (B) Same as (Ai) for an opsin-free control animal. (Ci,ii,D) Same as (Ai,ii,B) for sinusoidal light stimulation. (Ei,ii,F) Same as (Ai,ii,B) for ramp light stimulation.