Fig. 1.

Effects of Ffh and FtsY depletion on membrane insertion. (A and D) Growth of Ffh (A) and FtsY (D) depletion strains in NZA rich medium with or without 0.2% arabinose. (A and D Insets) Western blot with anti-Ffh or FtsY antibody. Samples were taken after 3 h of growth in medium with or without 0.2% arabinose. (B, C, E, and F) Biotinylation of MalF274-PSBT (B and E) or FtsQ-PSBT (C and F). Cells either were depleted (no arabinose) or contained Ffh or FtsY (0.2% arabinose). Expression of reporter proteins was either not induced or induced with 10 or 100 μM IPTG. Samples were taken after 1 h of induction for Western blot analysis by using streptavidin-AP. BCCP, biotin carboxyl carrier protein.