Fig. 2.

Effects of SecA depletion on translocation. (A) Growth of SecA depletion strain in NZA medium with (▪) or without (▴) 100 μM IPTG. (Inset) Western blot with an anti-SecA antibody. Samples were taken after 7 h of growth in medium with or without IPTG. (B) Biotinylation of AP-PSBT. Cells containing pTHS19 and a plasmid producing AP-PSBT were either depleted (no IPTG) or contained SecA (IPTG). Expression of AP-PSBT was not induced. Samples were taken after 6.5 h of growth for Western blot analysis with streptavidin-AP. BCCP, biotin carboxyl carrier protein. (C) Biotinylation of MalF274-PSBT was carried out as in B. (D) Biotinylation of FtsQ-PSBT was done as in B except that cultures were induced with 0.2% arabinose.