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Circular dichroism spectra for triple helical peptides COL-CPP (A) and PTX-COL-CPP (B). Thermal unfolding (white) shows that the peak at 224 nm is eliminated in a cooperative transition that indicates the presence of a triple helix. The first derivative of molar ellipticity (black) is used to indicate the temperature of helix-to-coil transition (Tm).
