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. Author manuscript; available in PMC: 2017 Aug 14.
Published in final edited form as: Cell. 2016 Dec 1;167(6):1481–1494.e18. doi: 10.1016/j.cell.2016.11.013

Figure 1. Slc7a5 mediates BCAA flux at the BBB.

Figure 1

(A) Representative images showing Slc7a5 (green) localization at the BBB in control animals (Slc7a5fl/fl, left) and its complete deletion in endothelial cells of the BBB in Cre positive mice (Tie2Cre;Slc7a5fl/fl, right). Immunostainings were performed in cortical slices at embryonic day 14.5 (E14.5 top), postnatal day 2 (P2, middle) and adulthood (>P40, bottom). Nuclei were stained with DAPI (blue). (B–D) Brain amino acid levels in Tie2Cre;Slc7a5fl/fl mice at E14.5 (B), P2-14 (C) and >P40 (D). Levels of amino acids were normalized on protein concentration and shown as fold change (log2 transformed) to levels in age-matched controls. In red are represented the amino acids with a fold change >1.3 and P value <0.05 (n>4 mice/genotype/time point).

See also Figure S1, S2 and Table S1.