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. Author manuscript; available in PMC: 2017 Aug 14.
Published in final edited form as: Biochem Biophys Res Commun. 2013 Jul 17;438(1):84–89. doi: 10.1016/j.bbrc.2013.07.030

Fig. 1.

Fig. 1

Generation of CD19-CAR modified T-cells using RetroNectin/anti-CD3 stimulation. (A) Schematic structure of SFG-1928z retroviral vector. VH and VL: variable heavy- and light chain; SD: splice donor; SA: splice acceptor; LTR: long terminal repeat; Gray box: CD8α signal peptide; Black box: (GGGGS)3 linker. PBLs were stimulated with RetroNectin/anti-CD3 and transduced twice with SFG-1928z retroviral vectors. They were propagated on CD19+ feeder cells. (B) Surface expression of CD19-CAR on transduced or non-transduced T-cells was examined by flow cytometry. All numbers show the percentages of CD3+ CD19-CAR+ populations. Representative data was shown in three independent experiments. (C) CD19-CAR expression in cell lysates from transduced T-cells or the control non-transduced T-cells was detected by Western blotting with anti-CD3ξ; β-actin is a loading control.