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. 2017 Jul 18;6:194–206. doi: 10.1016/j.omtm.2017.07.002

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© 2017 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Analysis of Baculovirus Transgene Expression upon shRNA Lentivirus Clones Acting on Antiviral Genes

Shown are representative images resulting from knockdown of antiviral genes by five independent shRNA clones targeting different regions of a gene. For simplicity, only a few genes are shown. Nuclei were stained with Hoechst 33342 (blue). (A1–A5) TLR2. (B1–B5) TLR3. (C1–C5) TLR4. (D1–D5) TLR9. (E1–E5) RIP1. (F1–F5) RIP2. (G1–G5) MyD88. (H1–H5) TRAF6. (I1–I5) TBK1. (J1–J5) PIK3CG. (K1–K5) POLR1D. (L1) shCtrl, negative control. (L2) Cell-only control. (L3) shTLR2, positive control.