Figure 7.

Necrostatin-1 Promotes Baculovirus Transgene Expression in RIP1 Knockdown Cells

(A and B) A549-shRIP1 or A549-shCtrl cells were cultured in medium containing progressively increasing concentrations of Necrostatin-1 (Nec-1) (A) or the HDAC inhibitor sodium butyrate (NaBu) (B), as indicated, for 24 hr and then transduced with vAcL at MOI = 50. Luciferase activities at 48 hpt were measured by a luminometer. Percentages of viable cells were measured by incubating with 10% v/v AlamarBlue for 1 hr, and the AlamarBlue intensity in each well was measured with a fluorescence reader. All experiments were performed three times independently, and the data shown are of one representative transduction experiment.