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. 2017 Jul 27;2017:5950395. doi: 10.1155/2017/5950395

Figure 5.

Figure 5

The effects of IL-17 on ALP activity and ALP mass of primary osteoblasts. (a) ALP activity in primary osteoblasts cultured with BMP-2 (300 ng/ml) in the presence of IL-17 (25 ng/ml), IL-22 (25 ng/ml), or IL-23 (25 ng/ml) for 7 days. ∗∗∗p < 0.001 compared with untreated control; #p < 0.05 compared with the cells stimulated with BMP-2 alone. (b) Staining for ALP in primary osteoblasts cultured with BMP-2 (300 ng/ml) in the presence of IL-17 (25 ng/ml), IL-22 (25 ng/ml), or IL-23 (25 ng/ml) for 7 days. (c) ALP activity in primary osteoblasts cultured with BMP-2 (300 ng/ml) in the presence of different concentrations of IL-17 (0, 5, 25, and 125 ng/ml) for 7 days. ∗∗p < 0.01 compared with untreated control; #p < 0.05 compared with the cells stimulated with BMP-2 alone. (d) Staining for ALP in primary osteoblasts cultured with BMP-2 (300 ng/ml) in the presence of different concentrations of IL-17 (0, 5, 25, and 125 ng/ml) for 7 days.