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. Author manuscript; available in PMC: 2018 Aug 4.
Published in final edited form as: J Mol Biol. 2017 Jul 10;429(16):2474–2489. doi: 10.1016/j.jmb.2017.07.002

Figure 4. Electron micrographs of assemblies produced by E153 mutants.

Figure 4

Undiluted pellet fractions from experiments shown in Figure 3 were mixed thoroughly, applied directly onto grids, rinsed, and stained with 1% uranyl acetate. A. E153Q B. E153A C. E153R D. E153V E. Example of the rare proheads found in the PEG fraction of mutant E153R. F. Narrow tubes and proheads produced by mutant E153H.