Skip to main content
. Author manuscript; available in PMC: 2018 Aug 4.
Published in final edited form as: J Mol Biol. 2017 Jul 10;429(16):2474–2489. doi: 10.1016/j.jmb.2017.07.002

Figure 5. Gel analysis of double and single mutants modifying the E153-R210E interaction.

Figure 5

A. Agarose gel. Double mutants E153R-R210E and E153Q-R210E and single mutant controls failed to assembly correctly. For mutants containing E153R, E153Q or R210E, the majority of the protein was found in the wells of the pellet fraction. E153R, which produced faint prohead and head-like bands. E153D and control K92A produced proheads and heads. B. SDS gel. Analysis of pellet fractions of double mutants showed results similar to the E153 mutants shown in Figure 3, but nearly all of the major capsid protein was cleaved for E153D, indicating normal assembly.