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. Author manuscript; available in PMC: 2018 Aug 4.
Published in final edited form as: J Mol Biol. 2017 Jul 10;429(16):2474–2489. doi: 10.1016/j.jmb.2017.07.002

Figure 6. Sheets and proheads made by E153 and R210 mutants.

Figure 6

Electron micrographs of pellet fractions from experiments shown in Figure 5 were prepared as described in the legend to Figure 4. A. R210E. Inset: Simulated diffraction pattern of area boxed on right (using FFT (fast Fourier transform) function in ImageJ [51]) showing a pattern typical for a hexagonal array with the lattice constant indicated. B. R210E/E153R and C. R210E/E153Q. The rest of the samples were diluted from purified stocks and stained as above. D. E153 Prohead II. E. WT Prohead II. F. WT Prohead I.