Figure 4.

Fine mapping of S40. (a) The physical map of S40. S40 was delimited to the interval flanked by ES-2 and ES-60 based on recombination events among F₂ progeny (202 +16 600 plants) derived from RD23/NIL-S40. (b) The key recombinant individuals for fine mapping from the F₂ population (left) and results of the corresponding F_2:3 reconfirmation (right). F, male and female full fertility and SS, male and female semi-sterility. (c) Eight ORFs were predicated by Gramene system in the 80 kb DNA fragment of cv. RD23.