Table 2. Segregation patterns of S40 locus for male and female gametes in the BC1F1 populations or F2 population.
Genetic crosses |
Progeny phenotype |
Expected segregation ratio | P-value | TRDk-value | ||||
---|---|---|---|---|---|---|---|---|
Female phenotype | Male phenotype | S40i/S40i | S40i/S40l | S40l/S40l | Total | |||
S40i/S40i | S40i/S40l | 118 | 122 | 240 | 1:1 | 0.8 | km=0.51 | |
S40i/S40l | S40i/S40i | 96 | 91 | 187 | 1:1 | 0.71 | kf=0.49 | |
S40i/S40l | S40,i/S40l | 89 | 184 | 95 | 368 | 1:2:1 | 0.91 |
Abbreviation: TRD, transmission ratio distortion. i and l indicate indica and longistaminata, respectively.
All homozygotes, S40iS40i and S40lS40l, were fertile (both pollen and spikelet), while the heterozygotes S40iS40l demonstrated typical pollen and embryo sac semi-sterility; P-value was obtained using the χ2-test under the hypothesis of Mendelian segregation.