Figure 2.

PQ induces release of Cyt-c in mouse AT-II cells. (A) Mouse AT-II cells were treated with 600 μM PQ for 8 h. Cyt-c was visualized by staining with anti-Cyt-c antibody, followed by secondary antibody. The mitochondria were visualized by Mito-Green staining and were observed under a confocal fluorescence microscope. (Images on the right side are magnified views of the boxed areas on its left side. Scale bar: 20 μm) (B) Mouse AT-II cells were treated with 600 μM PQ for 0, 4 and 8 h. The cells with released Cyt-c were counted. The values are represented the means ± SD of three independent experiments (at least 100 cells per experiment). ** P < 0.01, vs 0 h group.