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(A) Representative in vivo 1H MR spectrum demonstrating that the total choline signal cannot be spectrally resolved in vivo. (B) Representative 1H MRSI data set showing the spatial distribution of tCho displayed in red within an MDA-MB-231 tumor as described in the experimental section. (C) High-resolution MR spectrum obtained from a representative water-soluble MDA-MB-231 cell extract resolving GPC, PC, and Cho spectrally.
