Figure 4.
Schematic representation of the MSH/Mtr/NADPH/Rv2466c/TP053 catalytic cycle. Rv2466c in its reduced form reacts with TP053, leading to the formation of uncharacterized compounds. Subsequently, disulfide bond Rv2466c is recycled by the MSH/Mtr/NADPH pathways. The kcat value in the presence of MSH/Mtr/NADPH is in a good agreement with the values previously published in the presence of DTT and M. smegmatis methanol extract. However, the kobs for DTT and methanol extract is 80-fold faster than the value with MSH/Mtr/NADPH (17).