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. 2017 Jun 16;292(32):13122–13132. doi: 10.1074/jbc.M116.765941

Figure 2.

Figure 2.

Cry6Aa1 WCR forms pores in planar lipid bilayers. A, representative single channel current traces of Cry6Aa1 WCR at various holding voltages. 100 fg/ml of protein was added in the cis side compartment of the PLB setup and channel activity was recorded after a few minutes under symmetrical (150 mm KCl) conditions at pH 5.5. The traces were filtered at 1 kHz. B, typical current-voltage relationship graph (IV curves) constructed from data of one Cry6Aa1 WCR experiment under symmetrical conditions. The conductances ranged from 28 to 459 pS. C, representative single channel current traces of Cry6Aa1 WCR at ±60 and 0 mV under asymmetrical (450:150 mm KCl cis:trans) conditions at pH 5.5. D, IV curves of one single type of channel observed in each of three experiments conducted under asymmetrical (450:150 mm KCl cis:trans) conditions. The reversal potential was −11.39 mV ± 0.80 (n = 3). In A and C, the letters c and the dotted lines show the closed state of all channels.