Figure 3.

Both native Cry6Aa1 and Cry6Aa1 TT form pores in PLBs. A, representative traces of channel currents recorded under symmetrical (150 mm KCl) conditions at pH 5.5 at ±40 mV for the native Cry6Aa1 toxin (two upper traces) and at ±60 mV for Cry6Aa1 TT (two lower traces). Traces were filtered at 1 kHz. B, representative current traces of Cry6Aa1 at pH 5.5, 7.5, and 9.5. The holding voltage was −60 mV. The trace at pH 5.5 was unfiltered. The traces at pH 7.5 and 9.5 were filtered at 1 kHz. The letters c and the dotted lines indicate the closed state of all channels.