Table 2.
Bimolecular rate constants (k) of thiol-disulfide exchange reactions between apocyt c1, CcmG, and CcmH
| apocyt c1Cys-34-TNB | apocyt c1Cys-37-TNB | CcmH Cys-42-TNB | CcmH Cys-45-TNB | |
|---|---|---|---|---|
| CcmGCys-75 | 6.7a | 2.7 | 0.15 | 18.0 |
| CcmGCys-78 | 4.4 | 1.7 | 0.22 | 23.0 |
| CcmHCys-42 | 2.2 | 1.1 | NDb | ND |
| CcmHCys-45 | 2.9 | 1.8 | ND | ND |
| apocyt c1Cys-34 | ND | ND | 0.50 | 4.3 |
| apocyt c1Cys-37 | ND | ND | 0.67 | 2.3 |
a The reduced single Cys mutant derivatives of CcmG, CcmH, or apocyt c1 were reacted with single Cys mutant derivatives of apocyt c1–TNB and CcmH–TNB adducts, and their bimolecular reaction rate constants k (102 m−1 s−1) were determined as described under “Experimental procedures.”
b ND means not determined.