Figure 5.

JQ1 prevents BRD4 recruitment to the lytic origins of replication. A, EBV DNA content based on deep sequencing of chromatin from Akata-Zta cells treated with JQ1. Results are representative of two independent biological replicates. B, ChIP-seq mapping of BRD4 occupancy on the EBV genome in Akata-Zta cells treated with JQ1. Results are representative of two independent biological replicates. All conditions include acyclovir pretreatment and reactivation with doxycycline. Cells containing lytic EBV were purified based on positive LNGFR expression. For the EBV genome, locations of the BZLF1 gene and the left and right OriLyt elements are indicated. Occupancy is calculated as counts per million mapped reads.