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. 2017 Jun 21;292(32):13345–13360. doi: 10.1074/jbc.M117.795088

Figure 8.

Figure 8.

Effects of S1191L/V1197A on CFH. A, an SPR experiment was performed in a manner similar to that shown in Fig. 2B to compare the affinity (for immobilized C3b) of LA-CFH (left) with that of I62-CFH (right; see experiment 1 in Table 1). This experiment was repeated with similar results (see experiment 2 in Table 1). B, an SPR-based assay of the DAA of LA-CFH was performed in a manner similar to those shown in Fig. 3 except that 40 nm CFH solutions were used. The data shown are representative of two experiments. C, an ES-based assay of DAA was carried out in a manner similar to that shown in Fig. 5A. D, an ES-based assay of CA was carried out in a manner similar to that shown in Fig. 5B. E, in an ES-surface assay (see Fig. 5A), PspCN (2:1 to LA-CFH) does not restore any DAA to LA-CFH. F, conversely, in an ES-surface assay (see Fig. 5B), PspCN (2:1 to LA-CFH) significantly enhanced the CA of LA-CFH. For C–F, mean and S.D. (error bars) of triplicate measurements are plotted, and sigmoidal curves were fitted using SigmaPlot. Each graph is representative of two experiments.