Table 1.

Summary of SPR data

Experiment	Variant	Ligand	Without PspCN				With PspCN
			KD (μm)a	Rmax	Roffset	χ2	KD (μm)	Rmax	Roffset	χ2
I62-CFH versus V62-CFH versus plasma CFH	I62-CFH	C3b	0.21 ± 0.02	79.2	32.2	5.2
	V62-CFH	C3b	0.27 ± 0.03	80.0	19.3	4.6
	plasma-CFH	C3b	0.33 ± 0.02	59.6	8.0	0.6
	I62-CFH	C3d	1.8 ± 0.2	50.6	0.5	0.3
	V62-CFH	C3d	2.1 ± 0.2	52.2	0.0	0.2
	plasma-CFH	C3d	high
I62-CFH versus mutants, with/without PspCN (Expt. 1)b	I62-CFH	C3b	1.2 ± 0.2	175	34.8	5.2	0.50 ± 0.07	266	63.5	31
	R53H-CFH	C3b	1.8 ± 0.3	217	42.9	6.5	1.0 ± 0.1	310	75.3	13
	LA-CFH	C3b	0.83 ± 0.10	181	46.6	8.3	0.70 ± 0.07	249	91.2	16
I62-CFH versus mutants, with/without PspCN (Expt. 2)	I62-CFH	C3b	1.0 ± 0.2	117	41.2	4.0	0.62 ± 0.08	132	55.6	5.8
	R53H-CFH	C3b	1.2 ± 0.1	152	41.1	3.7	0.58 ± 0.04	134	52.1	2.5
	LA-CFH	C3b	0.86 ± 0.11	146	45.7	6.6	0.60 ± 0.07	151	61.4	8.0

^a Shown are the S.E. values for the fits of the data from each experiment.

^b Expt., experiment.