Table 2.
Decay-accelerating and cofactor activities on sheep erythrocytes
| Experiment | CA IC50 (nm)a | Effect of the variation (or of PspCN)b | DAA IC50 (nm)a | Effect of the variation (or of PspCN)b |
|---|---|---|---|---|
| I62-CFH versus | 8.00 ± 0.33 | 1.19 ± 0.12 | 1.35 ± 0.10 | 0.94 ± 0.11 |
| V62-CFH | 9.50 ± 0.57 | 1.27 ± 0.05 | ||
| I62-CFH versus | 8.00 ± 0.33 | (0.48 ± 0.03) | 1.77 ± 0.05 | (0.86 ± 0.05) |
| I62-CFH + PspCN | 3.82 ± 0.06 | 1.52 ± 0.05 | ||
| I62-CFH versus | 17.5 ± 0.8 | 2.44 ± 0.26 | 1.41 ± 0.44 | 4.72 ± 0.32 |
| R53H-CFH | 42.7 ± 2.5 | 6.65 ± 0.25 | ||
| R53H-CFH versus | 42.7 ± 2.5 | (0.40 ± 0.04) | 6.68 ± 0.6 | (0.91 ± 0.15) |
| R53H-CFH + PsPCN | 17.2 ± 0.6 | 6.06 ± 0.05 | ||
| I62-CFH versus | 12.0 ± 0.8 | >40 | 1.33 ± 0.09 | 8.06 ± 1.31 |
| D1119G-CFH | >500 | 10.7 ± 1.05 | ||
| D1119G-CFH versus | >500 | 9.21 ± 0.54 | (1.24 ± 0.15) | |
| D1119G-CFH + PsPCN | >500 | 11.5 ± 0.7 | ||
| I62-CFH versus | 17.5 ± 2.6 | 3.63 ± 1.35 | 1.59 ± 0.06 | 1.83 ± 0.14 |
| LA-CFH | 63.4 ± 14 | 2.92 ± 0.12 | ||
| LA-CFH versus | 63.4 ± 14 | (0.75 ± 0.38) | 2.23 ± 0.16 | (0.87 ± 0.14) |
| LA-CFH + PsPCN | 47.5 ± 13 | 1.94 ± 0.17 |
a Shown are the S.E. values for the fits of the data from each experiment.
b The ratio of either IC50 for the variant/IC50 for I62-CFH or (in parentheses) IC50 for CFH with PSPCN/IC50 for CFH without PspCN.