Figure 4.

Chromatographic analysis of the parallel dimer formation mediated by the N–N interaction. Shown are size exclusion chromatography (Superdex 75 10/300 GL column) elution profiles of the human β4ex after 0–12 h of incubation at 20 °C (top panel) and SDS-PAGE analysis of the fractions eluted from size exclusion chromatography after 3 h of incubation (bottom panel). The incubation was performed under non-reducing conditions, and the chromatography and SDS-PAGE analysis were performed under reducing conditions.