Figure 2.

Verification of the quantitative method by A3B mutant activity. Plasmids encoding A3B wild type or point mutation were co-transfected with HBV into HepG2 cells. HBV DNA was extracted after 2 days of transfection and was amplified by 94 °C PCR. A, A3B mutational activity on the HBV DNAs was determined by pe1674 with the 94 °C PCR. The products of primer extension were separated by an 8% sequencing gel and quantitated with a PhosphorImager. B, A3B mutational activities reflected by the mutation frequencies are presented graphically. Each bar represents the average of triplicates for each treatment. C, A3B mutant protein expression analyses. A3B mutants were transfected into HepG2 cells, and the whole cell proteins were extracted after 2 days of transfection. A3B in the cell lysates were analyzed by Western blotting with an antibody against native A3B.