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. 2005 Mar 14;102(12):4506–4511. doi: 10.1073/pnas.0500830102

Table 1. Mutations in the analyzed genes in lymphoma of AID Tg mice.

Gene (region, GC composition) Cell type Mutations, n Total bases, n Mutated clones, n (total) Mutation frequency RGYW mutation, n (total) GC mutations, %
c-myc (exon 1 and intron 1, 54.6% GC) Lymphoma 1* 24 18,183 19 (19) 13.2 19 (24) 95.6
Lymphoma 2 68 22,968 24 (24) 29.6 24 (68) 83.5
Lymphoma 3 19 18,183 14 (19) 10.5 9 (19) 90.1
Lymphoma 4 9 18,183 11 (19) 4.95 6 (9) 88.7
Lymphoma 5 16 16,269 13 (17) 9.83 9 (16) 82.1
WT 0 20,097 0 (21) <0.50
c-myc (exon 2, 61.1% GC) Lymphoma 1 4 13,752 11 (18) 2.91 4 (4) 100
Lymphoma 2 5 15,280 16 (20) 3.27 1 (5) 50.8
Lymphoma 3 11 15,280 13 (20) 7.20 2 (11) 68.6
Lymphoma 4 9 11,460 14 (15) 7.85 3 (9) 68.6
Lymphoma 5 8 14,516 13 (19) 5.51 3 (8) 60.2
WT 1 15,280 1 (20) 0.65 0 (1) 0
pim1 (exons 1-4, 66.6% GC) Lymphoma 1 2 10,800 12 (14) 1.85 1 (2) 100
Lymphoma 2 3 13,680 3 (19) 2.19 1 (3) 33
Lymphoma 4 3 15,120 12 (21) 1.98 2 (3) 25
WT 0 14,400 0 (20) <0.70
atm (exon 1 and intron 1) Lymphomas 1, 2, and 4 0 5,330 0 (10) <1.88
p53 (exon 1 and intron 1) Lymphomas 1, 2, and 4 0 10,920 0 (10) <0.92
tgfbr2 (exon 1 and intron 1) Lymphomas 1, 2, and 4 0 6,660 0 (10) <1.50
k-ras (exon 1 and intron 1) Lymphomas 1, 2, and 4 0 4,620 0 (20) <2.16

Shown are the regions of gene sequences analyzed. Mutations are counted according to the presumed genealogic trees showing the relationship between the mutations (24). The same mutation is counted as a single event; thus, the present mutation frequency should be lower than that by direct sequence of the genomic PCR products in ref. 5. In our study, the PCR error rate was at <2.09 × 10-5. The mutation frequency was calculated from the number of mutations per the total bases analyzed × 10-4. The number of the mutations occurring on the RGYW/WRCY motifs (where R is A or G, Y is C or T, and W is A or T) are shown. Also shown are the relative mutation frequencies of GC bases after correction for base composition in the genes. —, not applicable.

*

Taken from ref. 24.

Statistical significance (P < 0.05) was determined by using a χ2 test.