Table 1. Mutations in the analyzed genes in lymphoma of AID Tg mice.
Gene (region, GC composition) | Cell type | Mutations, n | Total bases, n | Mutated clones, n (total) | Mutation frequency | RGYW mutation, n (total) | GC mutations, % |
---|---|---|---|---|---|---|---|
c-myc (exon 1 and intron 1, 54.6% GC) | Lymphoma 1* | 24 | 18,183 | 19 (19) | 13.2 | 19 (24)† | 95.6 |
Lymphoma 2 | 68 | 22,968 | 24 (24) | 29.6 | 24 (68)† | 83.5 | |
Lymphoma 3 | 19 | 18,183 | 14 (19) | 10.5 | 9 (19)† | 90.1 | |
Lymphoma 4 | 9 | 18,183 | 11 (19) | 4.95 | 6 (9)† | 88.7 | |
Lymphoma 5 | 16 | 16,269 | 13 (17) | 9.83 | 9 (16)† | 82.1 | |
WT | 0 | 20,097 | 0 (21) | <0.50 | — | — | |
c-myc (exon 2, 61.1% GC) | Lymphoma 1 | 4 | 13,752 | 11 (18) | 2.91 | 4 (4)† | 100 |
Lymphoma 2 | 5 | 15,280 | 16 (20) | 3.27 | 1 (5) | 50.8 | |
Lymphoma 3 | 11 | 15,280 | 13 (20) | 7.20 | 2 (11) | 68.6 | |
Lymphoma 4 | 9 | 11,460 | 14 (15) | 7.85 | 3 (9) | 68.6 | |
Lymphoma 5 | 8 | 14,516 | 13 (19) | 5.51 | 3 (8) | 60.2 | |
WT | 1 | 15,280 | 1 (20) | 0.65 | 0 (1) | 0 | |
pim1 (exons 1-4, 66.6% GC) | Lymphoma 1 | 2 | 10,800 | 12 (14) | 1.85 | 1 (2) | 100 |
Lymphoma 2 | 3 | 13,680 | 3 (19) | 2.19 | 1 (3) | 33 | |
Lymphoma 4 | 3 | 15,120 | 12 (21) | 1.98 | 2 (3) | 25 | |
WT | 0 | 14,400 | 0 (20) | <0.70 | — | — | |
atm (exon 1 and intron 1) | Lymphomas 1, 2, and 4 | 0 | 5,330 | 0 (10) | <1.88 | — | — |
p53 (exon 1 and intron 1) | Lymphomas 1, 2, and 4 | 0 | 10,920 | 0 (10) | <0.92 | — | — |
tgfbr2 (exon 1 and intron 1) | Lymphomas 1, 2, and 4 | 0 | 6,660 | 0 (10) | <1.50 | — | — |
k-ras (exon 1 and intron 1) | Lymphomas 1, 2, and 4 | 0 | 4,620 | 0 (20) | <2.16 | — | — |
Shown are the regions of gene sequences analyzed. Mutations are counted according to the presumed genealogic trees showing the relationship between the mutations (24). The same mutation is counted as a single event; thus, the present mutation frequency should be lower than that by direct sequence of the genomic PCR products in ref. 5. In our study, the PCR error rate was at <2.09 × 10-5. The mutation frequency was calculated from the number of mutations per the total bases analyzed × 10-4. The number of the mutations occurring on the RGYW/WRCY motifs (where R is A or G, Y is C or T, and W is A or T) are shown. Also shown are the relative mutation frequencies of GC bases after correction for base composition in the genes. —, not applicable.
Taken from ref. 24.
Statistical significance (P < 0.05) was determined by using a χ2 test.