Table 5. Mutations in cd4 and cd5 in lymphoma of AID Tg mice.
| Gene (region, GC composition) | Cell type | Mutations, n | Total bases, n | Mutated clones, n (total) | Mutation frequency | RGYW mutation, n (total) | GC mutations, % |
|---|---|---|---|---|---|---|---|
| cd4 (5′ side of intron 1, 43.1% GC) | Lymphoma 1 | 10 | 10,920 | 8 (13) | 9.16 | 1 (8) | 38.3 |
| Lymphoma 3 | 20 | 7,560 | 9 (9) | 26.5 | 4 (20) | 31.6 | |
| Lymphoma 6 | 16 | 8,400 | 10 (10) | 19.1 | 2 (16) | 51.1 | |
| cd5 (exon 1 and 5′ side of intron 1, 53.6% GC) | Lymphoma 1 | 11 | 8,400 | 6 (10) | 13.1 | 3 (11) | 39.3 |
| Lymphoma 3 | 14 | 8,400 | 9 (9) | 16.6 | 8 (14)* | 100 |
Shown are the regions of gene sequences analyzed. The mutation frequency was calculated from the number of mutations per the total bases analyzed × 10-4. The number of the mutations occurring on the RGYW/WRCY motifs (where R is A or G, Y is C or T, and W is A or T) are shown. Also shown are the relative mutation frequencies of GC bases after correction for base composition in the genes.
*
Statistical significance (P < 0.05) was determined by using a χ2 test.