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Proposed scheme for N(6)-modifications to Lys199 of human serum albumin (HSA) (I). In the current study, formyl adduct (III) was observed in all human biospecimens. Putative cyclization of formaldehyde involving Cys200 (Va) and self-cyclization of Lys199 (Vb) are postulated from MS/MS spectra obtained from blood incubated with formaldehyde.
