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. Author manuscript; available in PMC: 2018 Aug 15.
Published in final edited form as: Arch Biochem Biophys. 2017 Jun 16;628:42–56. doi: 10.1016/j.abb.2017.06.003

Fig. 4. Effect of site-specific ribose deuteration on the quality of the NMR spectra of an RNA thermometer.

Fig. 4

(A) secondary structure of the ‘core’ thermometer. (B) and (C) are 2D 1H–1H NOESY spectra recorded in D2O for completely protonated (B) or with H6/H8, H1′, H2′, D3′, D4′, D5′/D5′ and D5 ribose deuteration, respectively, at 25 °C and 600 MHz. Spectral simplification is remarkable.