Table 2.
Open field test statistical data
| Drug | Measurement | Treated | DMSO control | Welch’s t | df | p |
|---|---|---|---|---|---|---|
| (25 µM) | ||||||
| VPA | Distance (mm) | 287.6 ± 37.31 | 234.2 ± 29.23 | 1.127 | 32.16 | 0.268 |
| Center duration (s) | 91.93 ± 16.54 | 64.51 ± 17.93 | 1.124 | 33.78 | 0.269 | |
| Trazodone | Distance (mm) | 199.9 ± 29.26 | 198.0 ± 17.79 | 0.055 | 28.05 | 0.956 |
| Center duration (s) | 72.81 ± 21.17 | 85.73 ± 19.31 | 0.451 | 33.72 | 0.655 | |
| Diazepam | Distance (mm) | 138.5 ± 28.87 | 175.6 ± 26.73 | 0.941 | 33.8 | 0.354 |
| Center duration (s) | 73.25 ± 18.55 | 66.49 ± 18.03 | 0.261 | 33.97 | 0.796 | |
| Clemizole | Distance (mm) | 177.9 ± 32.12 | 107.5 ± 15.12 | 1.983 | 24.18 | 0.059 |
| Center duration (s) | 49.17 ± 18.93 | 97.65 ± 22.17 | 1.663 | 33.19 | 0.106 | |
| (250 µM) | ||||||
| VPA | Distance (mm) | 293.6 ± 52.82 | 172.2 ± 26.99 | 2.047 | 25.31 | 0.051 |
| Center duration (s) | 91.07 ± 20.05 | 78.36 ± 18.31 | 0.468 | 33.72 | 0.643 | |
| Trazodone | Distance (mm) | 168.8 ± 38.14 | 260.1 ± 43.95 | 1.57 | 33.34 | 0.126 |
| Center duration (s) | 128.82 ± 21.62 | 93.96 ± 21.30 | 1.149 | 33.99 | 0.259 | |
| Diazepam | Distance (mm) | 2.56 ± 2.04 | 73.06 ± 11.40 | 6.089 | 18.08 | <0.001 |
| Center duration (s) | 282.07 ± 16.59 | 59.73 ± 20.15 | 8.518 | 32.8 | <0.001 | |
| Clemizole | Distance (mm) | 36.89 ± 9.61 | 158.38 ± 32.62 | 3.572 | 19.93 | 0.002 |
| Center duration (s) | 83.29 ± 17.31 | 28.31 ± 6.61 | 2.967 | 21.85 | 0.007 |
Larval zebrafish (N = 18 per group) were pretreated one of four drugs at two concentrations (see Materials and Methods). Each treatment group was tested at the same time as a control group treated with DMSO. Drug-treated and control larvae were then placed in a novel arena and behavior was video recorded for 5 min. Both distance moved (mm) and duration spent in center of the arena (s) were measured for all larva. Table shows distance moved in mm (mean ± SEM), t value, degrees of freedom, and p value. Data from lower concentration tested (25 µM) is shown at top; 250 µM is shown below.