Table 1.

Cohort Description, Assay Pipeline, and Driver Gene Mutations in All Patients.^*

Patient	Age	Location Sampled	Clinical Stage	Whole-Exome Sequencing	Safe-Sequencing System	Targeted Amplicon Sequencing	Droplet Digital PCR Assay	Somatic Mutations Detected	Driver Gene Mutations
	Yr							no.
1	41	Paraovarian tissue	III	Yes	No	No	No	11	None
2	36	Sigmoid colon	III	Yes	No	No	No	0	None
3	25	Appendix	IV	Yes	Yes	No	No	5	PIK3CA
4	26	Right paraureteral serosa	III	Yes	No	No	No	0	None
5	29	Right bladder peritoneum	IV	Yes	No	No	No	0	None
6	34	Uterosacral peritoneum	IV	Yes	No	No	No	17	PPP2R1A
7	38	Bladder peritoneum	III	Yes	No	No	No	1	None
8	33	Appendix	IV	Yes	No	No	No	2	None
9	40	Colonic wall	IV	Yes	No	No	No	6	ARID1A
10	23	Sigmoid colon	IV	Yes	No	No	No	1	None
11	41	Rectum	IV	Yes	No	No	No	10	None
12	39	Appendix	IV	Yes	No	No	No	4	None
13	37	Ileum	IV	Yes	No	No	No	7	None
14	38	Paratubal soft tissue	III	Yes	No	No	No	1	None
15	42	Hypogastric peritoneum	IV	Yes	Yes	No	No	2	KRAS
16	39	Uterosacral ligament	III	Yes	No	No	No	0	None
17	49	Paraovarian tissue	III	Yes	No	No	No	1	None
18	47	Paratubal soft tissue	III	Yes	No	No	No	4	None
19	46	Paratubal soft tissue	III	Yes	No	No	No	2	None
20	44	Paratubal soft tissue	III	Yes	No	No	No	2	ARID1A
21	35	Abdominal wall	IV	Yes	No	No	No	1	None
22	51	Paratubal soft tissue	I	Yes	No	No	No	2	None
23	30	Paratubal soft tissue	II	Yes	No	No	No	1	None
24	48	Paratubal soft tissue	III	Yes	No	No	No	0	None
25	40	Rectovaginal tissue	II	No	No	Yes	Yes	1	KRAS
26	36	Rectovaginal tissue	III	No	No	Yes	Yes	1	KRAS
27	36	Ureter	II	No	No	Yes	No	0	None
28	50	Rectouterine pouch	IV	No	No	No	Yes	1	KRAS
29	27	Posterior cul-de-sac	III	No	No	No	Yes	1	KRAS
30	25	Uterosacral ligament	III	No	No	No	Yes	1	KRAS
31	33	Sigmoid colon	III	No	No	No	Yes	0	None
32	40	Uterosacral ligament	II	No	No	No	Yes	0	None
33	29	Uterosacral ligament	II	No	No	No	Yes	0	None
34	41	Rectovaginal tissue	IV	No	No	No	Yes	0	None
35	28	Sigmoid colon	IV	No	No	No	Yes	0	None
36	41	Peri ureter	IV	No	No	No	Yes	0	None
37	31	Uterosacral ligament	IV	No	No	No	Yes	0	None
38	34	Rectovaginal tissue	IV	No	No	No	Yes	0	None
39	29	Rectovaginal tissue	III	No	No	No	Yes	0	None

^*Tissue samples from Patients 1 through 17 were obtained from the Departments of Obstetrics and Gynecology and Pathology at Lenox Hill Hospital-Northwell Health (Hofstra University) in New York. Tissue samples from Patients 18 through 24 were obtained from the Department of Pathology at Seirei Mikatahara Hospital in Hamamatsu, Japan. Tissue samples from Patients 25 through 39 were obtained from the BC Women’s Centre for Pelvic Pain and Endometriosis in Vancouver, BC, Canada. PCR denotes polymerase chain reaction.