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. 2016 Jun 10;138(24):7603–7609. doi: 10.1021/jacs.6b02515

Figure 3.

Figure 3

(a) Urine samples (1 mL) from 14-week-old heterozygous (light gray bars) and Wilson’s disease (dark gray bars) mice analyzed by ICP-MS before (−) and after (+) exposure to 2 mg of PAF-1-SMe. (b) Absorption spectra after 8-hydroxyquinoline addition to dried PAF-1-SMe with DMSO washes applied to heterozygous (light gray) and Wilson’s disease (dark gray) urine specimens. (c) Correlation between direct copper measurements by ICP-MS (open circles) versus calculated copper levels from 410 nm light absorption using 8-hydroxyquinoline as an indicator (black filled squares). (d) Real time copper uptake of PAF-1-SMe in the urine samples of heterozygous (light gray) and Wilson’s disease mice (dark gray) measured at 1, 3, 5, 10, 20, and 30 min intervals and fitted with the double exponential decay model: y = A1 exp(−x/t1) + A2 exp(−x/t2); ⟨τWilson’s disease⟩ = 15.9 min and ⟨τHeterozygous⟩ = 5.4 min (red lines).