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. Author manuscript; available in PMC: 2017 Aug 14.
Published in final edited form as: Chem Res Toxicol. 2015 Jun 8;28(7):1398–1408. doi: 10.1021/acs.chemrestox.5b00015

Figure 4.

Figure 4

EPR spectra (X-band, 10 K) of mouse heart tissue. (A) EPR spectrum of minced heart tissue following dose of 24 mg/kg NaNO2 administered ip 5–10 min prior to sacrifice showing clear evidence for the generation of nitric oxide. Signal (4) at ~1100 gauss: metMb; signal (5) at ~3300 gauss: MbNO. The combined intensities of the metMb plus MbNO signals approach 100% of the total heme (~260 μM) present in the heart. (B) Spectrum of heart tissue following dose of 16 mg/kg NaHS. (C) Spectrum of heart tissue following dose of 24 mg/kg of NaNO2 (t = 0) and 16 mg/kg of NaHS at 2 min. No signals due to metMbSH were observed. (D) Spectrum of heart tissue following NaHS injection 2 min prior to NaNO2. (metHbSH signals not detected.)