Figure 6.

Resistance of bovine pulmonary artery endothelial cells (BPAEC) to sulfide toxicity is increased in the presence of sodium nitrite. Effect of NaNO₂ on BPAEC and BPAEC treated with NaHS. BPAEC were plated and then covered with Parafilm just prior to experiments. Aqueous solutions (in media) of both NaNO₂ (1 mM) and NaHS (5 mM) were injected through the Parafilm into the cell media of wells while shaking the plates gently. Plates were incubated at 37 °C for 1 h and then treated with both SYBR Green and propidium iodide dyes. Cell counts were taken using a Zeiss IM 35 fluorescent microscope and an Infinity 2 camera with the Infinity Analyze software.