Figure 1.

Isolation, cloning and characterization of amha and amhy in H. tsurugae. (A) Polymerase chain reaction-amplified amha in both male and female wild specimens (upper band) and amhy amplified only in males (lower band). (B) Comparison of full-length gene structure of amha and amhy in H. tsurugae. Compared to amha, the amhy gene of H. tsurugae is shorter, lacks exons II and III, and contains a specific insertion of 195 bp at the position of exons II and IV. Exon V is present in the genomic sequence but it is not transcribed. (C) Identity values of nucleotide sequence between amha and amhy exons, untranslated regions (UTRs), and the transforming growth factor-β (TGF-β) domain.