Table 4. Quantitative PCR of expression levels in cis-eQTL genes in B6 and D2 brain tissues.
Preseizure Exposure | Postseizure Exposure | ||||||
---|---|---|---|---|---|---|---|
Tissue | Gene | B6/D2 Ratio | Expression B6/D2a | P value | B6/D2 Ratio | Expression B6/D2a | P value |
Cerebellum | Camta1 | 0.82 | 0.92(0.1)/1.18(1.1) | 0.9508 | 0.83 | 1.0(0.14)/1.2(0.17) | 0.7964 |
Hippocampusb | Camta1 | 2.08 | 0.54(0.07)/0.28(0.02) | 0.0019 | 1.96 | 0.49(0.34)/0.25(0.08) | 0.0434 |
Cerebellum | Park7 | 0.53 | 0.33(0.03)/0.62(0.16) | 0.9246 | 1.31 | 0.67(0.11)/0.51(0.19) | 0.1243 |
Hippocampus | Park7 | 1.63 | 0.36(0.06)/0.19(0.03) | 0.0317 | 1.84 | 0.31(0.23)/0.53(0.28) | 0.0903 |
Cerebellum | Per3 | 1.32 | 1.25(0.19)/0.95(0.12) | 0.1172 | 1.14 | 1.04(0.25)/0.91(0.16) | 0.344 |
Hippocampus | Per3 | 2.10 | 0.86(0.15)/0.41(0.07) | 0.0196 | 0.55 | 0.6(0.07)/0.54(0.19) | 0.9797 |
Cerebellum | Vamp3 | 0.46 | 0.51(0.02)/1.23(0.43) | 0.9051 | 1.40 | 1.25(0.37)/0.89(0.47) | 0.1967 |
Hippocampus | Vamp3 | 1.33 | 0.36(0.03)/0.27(0.06) | 0.1632 | 0.58 | 0.38(0.04)/0.66(0.15) | 0.93 |
Expression levels quantified using relative standard curve approach normalized to β actin mRNA levels (SEM).
Data listed in bold indicates P < 0.05.