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. 2017 Jun 17;7(8):2651–2664. doi: 10.1534/g3.117.043836

Figure 9.

Figure 9

Mean ± SEM of the ratio of low cSOD: high cSOD activity MEN activity standardized by protein concentration (micrograms per milliliter) for adult male and female flies across the eight DGRPi genetic backgrounds. Values closer to 1 indicate more similar enzyme activities at the levels of cSOD activity being compared, and C.I.s are at 95% and represent the ratio of the low cSOD activity and high cSOD activity SEMs. (A) 0% cSOD activity: 50% cSOD activity: Female: lowest ratio 313, 0.796 < 0.970 < 1.16; highest ratio 335, 1.09 < 1.28 < 1.50; Male: lowest ratio 705, 0.730 < 0.863 < 1.02; highest ratio 304 - 0.963 < 1.11 < 1.28. (B) 30% cSOD activity: 80% cSOD activity: Female: lowest ratio 705, 0.646 < 0.777 < 0.929, highest ratio 304, 0.762 < 1.18 < 1.68; Male: lowest ratio 705, 0.827 < 0.956 < 1.10; highest ratio 335, 1.14 < 1.38 < 1.68. ANOVA was used to test the factor effects with an α of 0.05. Partial eta squared (ηp2) was calculated to quantify the effect of each factor. (A) Sex-by-cSOD activity, F1,265 = 32.8, P < 0.0001, ηp2 = 0.110. (B) Sex-by-genetic background, F7,280 = 3.18, P = 0.003, ηp2 = 0.0736; Genetic background-by-cSOD activity, F7,280 = 3.16, P = 0.0031, ηp2 = 0.0733; sex-by-cSOD activity, F1,280 = 10.5, P = 0.0013, ηp2 = 0.0364. cSOD, cytosolic copper/zinc superoxide dismutase; DGRPi, ; MEN, malic enzyme.