Table 1.
Oligonucleotide sequences used in the Hot Ligation
| Description | Oligo Name | bp Positiona | Oligo sequence 5' – 3' | Modifications |
| Suspt. East kdr detector | Kdr104L-DTe | 311-15i | ATTTGCATTACTTACGACTA | 5' Biotin |
| Resist. East kdr detector | Kdr104S-DTe | 311-15i | ATTTGCATTACTTACGACTG | 5' Biotin |
| East kdr reporter | Kdr104-RTe | 291–310 | AATTTCCTATCACTACAGTG | 5' Phosphorylation 3' Fluorescein |
| Suspt. West kdr detector | Kdr104L-DTw | 312-16i | AATTTGCATTACTTACGACT | 5' Biotin |
| Resist. West kdr detector | Kdr104F-DTw | 312-16i | AATTTGCATTACTTACGACA | 5' Biotin |
| West kdr reporter | Kdr104-RTw | 292–311 | AAATTTCCTATCACTACAGT | 5' Phosphorylation 3' Fluorescein |
aUsing sequence from Martinez-Torres et al., as reference; i intron 2 position.