Table 1.
Stability of a sample transcript – full length target and sheared fragments Folding ΔG of target transcript and fragment molecules shown in Figure 8, at hybridization temperatures commonly used for long oligomer arrays.
| Molecule | 2G, kcal/mole | |||
| 42°C | 52°C | |||
| DNA | RNA | DNA | RNA | |
| 70-mer Probe | - 6.8 | N/A | - 4.2 | N/A |
| Full Length Target | - 85.9 | - 188.4 | -56.6 | - 140.2 |
| 200-mer sheared Target | - 25.5 | - 58.6 | -15.9 | - 41.6 |
| 100-mer sheared Target | -14.2 | - 25.7 | -9.6 | -18.0 |
| 50-mer sheared Target (not shown) | - 6.1 | -10.5 | - 4.2 | -7.3 |