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. Author manuscript; available in PMC: 2017 Aug 14.
Published in final edited form as: Methods Mol Biol. 2017;1570:1–15. doi: 10.1007/978-1-4939-6840-4_1

Fig. 2.

Fig. 2

Schematic representing the procedure to quantify siRNA duplexes on nanoparticles (NPs). (a) Antisense RNA strands (blue) are dehybridized from sense RNA strands (red) on nanoparticles by incubating in 8 M urea at 45 °C. The sense-loaded nanoparticles are pelleted by centrifugation, and the supernatant containing the antisense strands is collected. Antisense RNA strands within the supernatant are measured using components of the Quant-iT™OliGreen® kit. (b) Sense RNA strands (red) are removed from the nanoparticles’ surfaces by breaking the gold–thiol bond with β-Mercaptoethanol. The nanoparticles are pelleted by centrifugation, and the supernatant containing the sense RNA strands is collected for analysis of RNA content with the Quant-iT™ OliGreen® kit