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. Author manuscript; available in PMC: 2017 Aug 14.
Published in final edited form as: Biochem J. 2017 Jan 15;474(2):195–214. doi: 10.1042/BCJ20160516

Figure 6. Localization of AFs on the particles undergoing ITS2 spacer removal.

Figure 6

Cryo-EM structure of Nog2 (A) and Rix1 (B) particles representing two late nuclear intermediates. The Nog2 particles represent the earlier assembly intermediate in which the ITS2 spacer has not yet been removed and the 5S rRNP is still in a premature conformation. In wild-type Rix1 particles, ITS2 is removed and rotation of 5S rRNP is completed. Domain V of 25S rRNA containing the PTC (yellow), the subunit interface, and the PET of the pre-60S particles are bound by many AFs that help to construct and inspect the functional centers [57,129] (PDB IDs: 5FL8, 3JCT).