Table 1.
Statistics of structural determination and model refinement
| Pre-60S ribosomal particle (EMD-9569, PDB 5H4P) |
|
|---|---|
| Data collection | |
| Electron microscope | Titan Krios |
| Voltage (kV) | 300 |
| Electron detector | Falcon II camera |
| Electron dose (e−/Å2) | ~20 |
| Pixel size (Å) | 1.08 |
| Defocus range (µm) | 1.5–2.5 |
| Reconstruction and model refinement | |
| Particles for final refinement | 84,240 |
| Resolution of unmasked map (Å) | 3.4 |
| Resolution of masked map (Å) | 3.07 |
| Map-sharpening B factor (Å2) | –30 |
| R factor | 0.3198 |
| Fourier shell correlation | 0.7245 |
| Model composition | |
| Peptide chains | 44 |
| Residues | 9,809 |
| RNA chains | 3 |
| RNA bases | 3,377 |
| R.m.s. deviation | |
| Bond length (Å) | 0.0053 |
| Bond angle (°) | 0.9611 |
| Ramachandran plot | |
| Favored (%) | 88.80 |
| Outliers (%) | 2.47 |
| Validation (protein) | |
| MolProbity score | 2.01 (99th percentile) |
| Clashscore | 1.74 (100th percentile) |
| Good rotamers (%) | 85.91 |
| Validation (RNA) | |
| Correct sugar puckers (%) | 98.76 |
| Good backbone conformations (%) | 73.85 |