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. Author manuscript; available in PMC: 2017 Aug 14.
Published in final edited form as: J Thorac Oncol. 2016 May 24;11(9):1469–1481. doi: 10.1016/j.jtho.2016.05.010

Figure 2.

Figure 2

Inositol-triphosphate 3-kinase A gene (ITPKA) promotes tumorigenesis. (A) Western blot analysis of non–small cell lung cancer cells stably overexpressing ITPKA (left), and cells in which the expression of endogenous ITPKA was knocked down using small interfering RNA (siRNA) oligomers (oligo) (middle) and lentivirus-based short hairpin RNA (shRNA) approach (right). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) served as the loading control. (B) Cell proliferation rate was determined using cell counting kit-8 reagent. (C) Colony formation assay was performed by plating and maintaining cells for 12 days, and colony numbers were scored after crystal violet staining. (D) Cells were subcutaneously injected into NSG mice (n = 6 per group), and tumor volumes were monitored. HA, hemagglutinin.