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. Author manuscript; available in PMC: 2017 Aug 14.
Published in final edited form as: Anal Chem. 2017 Apr 17;89(12):6367–6376. doi: 10.1021/acs.analchem.7b00212

Figure 6. AI-ETD enables confident characterization of intact phosphoprotein α-casein.

Figure 6

a) AI-ETD provides the greatest sequence coverage of α-casein for all charge states investigated. b) The phosphor isoform of α-casein investigated had 8 phosphosites (out of nine previously reported sites). Only AI-ETD confidently localized all eight phosphosites for each precursor fragmented. The number of fragments (blue line) and the percentage of these fragments that are b- and y-type product ions (gold bars) are shown from ETD (c), EThcD (d), and AI-ETD (e).