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. 2017 Aug 14;12(8):e0183076. doi: 10.1371/journal.pone.0183076

Fig 1. Upregulation of heme oxygenase-1 (HO-1) following proteasome inhibition by MG132 in the substantia nigra of rat.

Fig 1

(A) MG132 (9.5 ng) was locally injected into the substantia nigra of rats, and the rats were sacrificed 24 h later. Immunofluorescent staining shows that HO-1 expression increased following MG132 treatment and HO-1 occurred mostly in TH (+) neurons. Scale bar: 100 μm. (B) Treatment with MG132 (1 μM) in cultured SH-SY5Y cells. Levels of ROS were analyzed using a DCF fluorescence assay. Note that the generation of ROS increased time-dependently after MG132-treatment. Results are expressed as percentage of wild-type control (mean ± S.E.M.) (n = 4).