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. 2017 Aug 14;12(8):e0183076. doi: 10.1371/journal.pone.0183076

Fig 2. PINK1 G309D mutation inhibits MG132-induced expression of HO-1 in SH-SY5Y cells.

Fig 2

(A) SH-SY5Y cells were treated with different concentrations of MG132 for 24 h. Note that MG132 concentration-dependently enhanced HO-1 protein expression. The representative blots are illustrated in the upper panel and the fold-increases measured relative to controls are shown in the lower panel. (B) Empty vector-transfected cells, WT-PINK1 cells and PINK1 G309D mutant cells were treated with MG132 (1 μM) for various time intervals. Note that MG132 time-dependently increased HO-1 protein levels in wild-type cells and overexpression of WT-PINK1 enhanced HO-1 production, whereas the level of HO-1 was impaired in PINK1 G309D mutant cells. The quantitative results are shown in (C). (D) The HO-1 mRNA levels were analyzed by real time-PCR after MG-132 treatment. Note that of HO-1 mRNA was time-dependently upregulated by MG132. In comparison with empty vector-transfected cells, the regulation of HO-1 mRNA levels induced by MG132 was potentiated in WT-PINK1 cells and inhibited by the expression of the recombinant PINK1 G309D mutant. *p < 0.05 compared with empty vector-transfected control cells (con 1); #p < 0.05 compared with the corresponding MG132-treated empty vector-transfected cells (con 2). %p < 0.05 compared with the correspondening MG132-treated empty vector-transfected cells (con 3). (ox: overexpression).