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Representative immunofluorescence micrographs depicting that the 47xCAG RNA foci co-localize with the marker for nuclear speckles (SC-35) but not with other nuclear bodies such as PML bodies (PML), paraspeckles (nmt55), nucleoli (Fib1) or Cajal bodies (coilin). RNA foci are stained using an antibody against GFP. Nuclei are stained with DAPI. Data are representative of 3 or more independent experiments. Scale bars are 5 μm.
