Figure 4. Nuclear foci are reversed by agents that disrupt RNA gelation in vitro.

(a, d) NH₄OAc (a) and doxorubicin (Dox) (d) prevent 47xCAG RNA clustering. (b) Images of the same cell before and 300 s after treatment with NH₄OAc, and corresponding quantification. (c, e, f) Representative images and quantification of RNA foci upon transfection with indicated ASOs (c), and upon Dox treatment in U-2OS cells expressing exogenous 47xCAG RNA (e) and DM1 fibroblasts (f). Scale bars, 5 μm. Error bars, median and interquartile range. Data are representative of ≥ 3 independent experiments.