FIGURE 3:

Single-molecule recovery of Pil1p-SiR occurs at eisosome filament ends. (A) Probability distributions of measured distances (black squares, 191 spot/filament pairs across 20 cells) and simulation results (uniform dynamics model, blue; end dynamics model, magenta; 275,000 runs for each tested model). The simulation models are illustrated in schematic form. For the end model simulations, the dashed line represents simulations using an unbiased Gaussian noise distribution for eisosome end localizations, and the solid line represents simulations using the true noise predicted from fitting a dynamic end (as in Figure 2D). (B) Table of mean and SD of distributions for simulated data sets and measured SRAP spots.